Kinetic studies of the polygalacturonase enzyme from Colletotrichum lindemuthianum
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چکیده
منابع مشابه
Kinetic studies of the polygalacturonase enzyme from Colletotrichum lindemuthianum.
The intrinsic protein fluorescence of the polygalacturonase from Colletotrichium lindemuthianum was exploited in stopped-flow experiments aimed at elucidating the kinetic mechanism for this enzyme. Binding of the polymeric substrate polygalacturonic acid (PGA) essentially produced a triphasic fluorescence profile. There was an initial rapid quench in fluorescence, consistent with the rapid form...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1992
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2850551